THP-1-EGFP cell line

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THP-1-EGFP Order

Catalog#

YC-D011-EGFP-P

Size

1*10^6

Transfection Culture Medium

Transfection Culture Medium--Suspension Cells

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

Order

Detailed Product Information

Product category

EGFP Stable Cell Lines

cell name

THP-1-EGFP

Cell Morphology

Monocyte,suspension

Passage ratio

1：2-1：4

Species

Human

Reporter gene

EGFP

Resistance gene

puro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90%RPMI-1640+10%FBS

Accession

CVCL_C8WG(Cellosaurus)

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:THP-1			STR Info (Cell bank) Cell Line:THP-1
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	13	13		13	13
D7S820	10	10		10	10
D16S539	11	12		11	12
VWA	16	16		16	16
TH01	8	9.3		8	9.3
AMEL	X	Y		X	Y
TPOX	8	11		8	11
CSF1PO	11	13		11	13
D12S391	19	19
FGA	24	25
D2S1338	17	18
D21S11	30	31.2
D18S51	13	14
D8S1179	10	14
D3S1358	15	17
D6S1043	14	14
PENTAE	11	15
D19S433	12.2	13
PENTAD	10	12
D1S1656	16	17

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

EGFP validation

Instruction

Use EGFP gene expressing lentivirus constructed by Ubigene to infect the THP-1 cell line， screen the cells based on the puromycin resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a THP-1-EGFP cell line that can maintain long-term, stable and high expression.

THP-1-EGFP

Catalog#

YC-D011-EGFP-P

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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THP-1-EGFP cell line

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